International Journal of Medical Investigation
Int J Med Invest
Medical Sciences
http://intjmi.com
1
admin
2322-2913
2345-640x
no
no
14
8888
13
en
jalali
1393
6
1
gregorian
2014
9
1
3
3
online
1
fulltext
en
Designing A Bacterial Biosensor For Arsenic Detection In Water Solutions
عمومى
General
پژوهشي
Research
In parallel to continuous development of increasingly more sophisticated physical and chemical
analytical technologies for the detection of environmental pollutants, there's a progressively more urgent
need also for bioassay which report not only on the presence of a chemical but also on its bioavailability
and its biological effects. As a partial fulfillment of that need, there's been a rapid development of
biosensors based on genetically engineered bacteria. Natural water was polluted by arsenic that known
toxin and carcinogen leaded us to designing a whole cell bacterial biosensor to detect arsenic in liquid
solutions.
Methods: In order to construct the biosensor, chromosomal arsR gene and its related promoter/operator
from Escherichia coli strain Bl21(DE3), gfp gene as the reporter, plasmid pUC19 as the basic vector and
different molecular and genetic engineering techniques such as PCR, gene cloning, etc. were applied to
make the Escherichia coli strain BL21(DE3) act as the arsenic whole cell biosensor. Arsenic detection by
using this biosensor was done by means of microscopy and fluorometery techniques. Strain BL21(DE3)
responded mainly to As(III) and As(V) with the lowest detectable concentration being 5 μM during a 3-
hours exposure and 1 and 3 μM respectively, with an 6-hours induction period.
Results: Our result demonstrate that the nonpathogenic bacterial biosensors developed in the present
study could be useful and applicable in determining the bioavailability of arsenic with high sensitivity in
contaminated water samples after further optimization, and they suggest a potential for its inexpensive
application in field-ready tests.
Conclusion: Our success in designing and producing this biosensor, in addition to localizing this useful
technology, will be a very large and effective step to improve the health status of drinking water, through
to speed up detection of arsenic contamination of water resources. It can also attract lots of academic and
industrial heads for research in this interesting field of science and establish an optimistic future in
developing biosensor technology in our country.
arsR, Arsenic Contaminant, Biosensor, GFP, Whole Cell Biosensor
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Maryam
Daneshpour
1003194753284600824
1003194753284600824
No
Nooshin
Shabab
1003194753284600825
1003194753284600825
No
Amir
Roointan
1003194753284600826
1003194753284600826
No
Alireza
Rahmani
1003194753284600827
1003194753284600827
No
Gholam Abbas
Chehardoli
1003194753284600828
1003194753284600828
No
Massoud
Saidijam
1003194753284600829
1003194753284600829
Yes