en عمومى General پژوهشي Research Listeria monocytogenes is an opportunistic intracellular pathogen that has become an important cause of human food borne infections worldwide. The aim of this study is the determinate sensitivity of diagnostic methods of this organism and achieving a test that can detect Listeria monocytogenes in all circumstances in clinical samples. Materials and Methods: In this study 88 vaginal swabs were collected from women with age of (15-45) referred to gynecologic clinics in the hospital (Sari, Iran). Vaginal swabs were examined by culture on specific PALKAM agar medium and PCR technique by specific primers. Results: Out of 88 studied vaginal swabs, 8 cases with culture method and 27 cases were positive with PCR technique. The sensitivity of PCR and culture is reported %100, %29 respectively. Conclusion: The results show that PCR is a more sensitive, easier, and faster method in comparison to in clinical samples. Listeria monocytogenes, PCR, culture methods 71 74 http://intjmi.com/browse.php?a_code=A-10-1-175&slc_lang=en&sid=1 Mohtaram Nasrollahi 10031947532846002799 10031947532846002799 No Professor in Microbiology, Department of Microbiology, Mazandaran University of Medical Science, Mazandaran, Sari, Iran. Zeynab Marzhoseyni 10031947532846002800 10031947532846002800 No MSc in Microbiology, Department of Microbiology, Mazandaran University of Medical Science, Mazandaran, Sari, Iran Tahoora Mousavi 10031947532846002801 10031947532846002801 No Ph.D. Student in Molecular and Cell Biology, Student Research Committee, Molecular and Cell Biology Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran. Mohammad Ahanjan 10031947532846002802 10031947532846002802 Yes Associate Professor, Department of Microbiology, Mazandaran University of Medical Science, Mazandaran, Sari, Iran.