International Journal of Medical Investigation
Int J Med Invest
Medical Sciences
http://intjmi.com
1
admin
2322-2913
2345-640x
no
no
14
8888
13
en
jalali
1399
9
1
gregorian
2020
12
1
9
4
online
1
fulltext
en
The Prevalence of Malassezia yeasts in patients with seborrheic dermatitis by
PCR-RFLP method in Isfahan, Iran
عمومى
General
پژوهشي
Research
Introduction: Malassezia spp. are lipophilic basidiomycetes that are the microbial members of the<br>
flora of most warm-blooded animals. The natural habitat of these lipophilic yeasts is the horny part of<br>
human and animal skin. Malassezia population densities in infected lesions are generally higher than<br>
in healthy skin, and yeast proliferation appears to be the first step in developing Malassezia dermatitis.<br>
Under certain conditions, these yeasts can cause diseases such as Pityriasis versicolor and seborrheic<br>
dermatitis, and even cause systemic infections. The aim of this study was to investigate the prevalence<br>
of Malassezia in Isfahan, Iran.<br>
Material and methods: In this descriptive cross-sectional study in 2011 in Isfahan, 100 people (70<br>
men and 30 women) with seborrheic dermatitis were studied. Sampling was performed by chipping<br>
from dandruff and behind the ears. The samples were studied microscopically. After culturing and<br>
extracting DNA from the colonies, Malassezia spp. were identified by PCR-RFLP method.<br>
Results: Based on the results, the total number of yeast cells in the samples of patients with seborrheic<br>
dermatitis was 53 (53%) and also 38 (38%) of yeast colonies grew. Based on PCR-RFLP results, five<br>
species including Malassezia globosa, Malassezia furfur, Malassezia restricta, Malassezia<br>
sympodialis and Malassezia slofiae were identified.<br>
Conclusion: Based on PCR-RFLP analysis in healthy control, seborrheic dermatitis and Pityriasis<br>
versicolor in patients referred to laboratories in Isfahan, Malassezia globosa was the most common<br>
isolated species among the five isolated species.Introduction: Malassezia spp. are lipophilic basidiomycetes that are the microbial members of the<br>
flora of most warm-blooded animals. The natural habitat of these lipophilic yeasts is the horny part of<br>
human and animal skin. Malassezia population densities in infected lesions are generally higher than<br>
in healthy skin, and yeast proliferation appears to be the first step in developing Malassezia dermatitis.<br>
Under certain conditions, these yeasts can cause diseases such as Pityriasis versicolor and seborrheic<br>
dermatitis, and even cause systemic infections. The aim of this study was to investigate the prevalence<br>
of Malassezia in Isfahan, Iran.<br>
Material and methods: In this descriptive cross-sectional study in 2011 in Isfahan, 100 people (70<br>
men and 30 women) with seborrheic dermatitis were studied. Sampling was performed by chipping<br>
from dandruff and behind the ears. The samples were studied microscopically. After culturing and<br>
extracting DNA from the colonies, Malassezia spp. were identified by PCR-RFLP method.<br>
Results: Based on the results, the total number of yeast cells in the samples of patients with seborrheic<br>
dermatitis was 53 (53%) and also 38 (38%) of yeast colonies grew. Based on PCR-RFLP results, five<br>
species including Malassezia globosa, Malassezia furfur, Malassezia restricta, Malassezia<br>
sympodialis and Malassezia slofiae were identified.<br>
Conclusion: Based on PCR-RFLP analysis in healthy control, seborrheic dermatitis and Pityriasis<br>
versicolor in patients referred to laboratories in Isfahan, Malassezia globosa was the most common<br>
isolated species among the five isolated species.
Malassezia spp, Pityriasis versicolor, Seborrheic dermatitis, MLNA culture medium,
PCR-RFLP.
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Maryam
Sarlak
10031947532846003801
10031947532846003801
No
Department of Microbiology, Islamic Azad University, Falavarjan Branch, Isfahan, Iran.
Pegah
Shakib
10031947532846003802
10031947532846003802
No
Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.
Mohammad Ali
Zia
10031947532846003803
10031947532846003803
No
Department of Basic Science, Islamic Azad University, Khorasgan Branch, Isfahan, Iran.
Mahboobe
Madani
10031947532846003804
10031947532846003804
Yes
Department of Microbiology, Islamic Azad University, Falavarjan Branch, Isfahan, Iran.