en عمومى General پژوهشي Research Introduction: Malassezia spp. are lipophilic basidiomycetes that are the microbial members of the<br> flora of most warm-blooded animals. The natural habitat of these lipophilic yeasts is the horny part of<br> human and animal skin. Malassezia population densities in infected lesions are generally higher than<br> in healthy skin, and yeast proliferation appears to be the first step in developing Malassezia dermatitis.<br> Under certain conditions, these yeasts can cause diseases such as Pityriasis versicolor and seborrheic<br> dermatitis, and even cause systemic infections. The aim of this study was to investigate the prevalence<br> of Malassezia in Isfahan, Iran.<br> Material and methods: In this descriptive cross-sectional study in 2011 in Isfahan, 100 people (70<br> men and 30 women) with seborrheic dermatitis were studied. Sampling was performed by chipping<br> from dandruff and behind the ears. The samples were studied microscopically. After culturing and<br> extracting DNA from the colonies, Malassezia spp. were identified by PCR-RFLP method.<br> Results: Based on the results, the total number of yeast cells in the samples of patients with seborrheic<br> dermatitis was 53 (53%) and also 38 (38%) of yeast colonies grew. Based on PCR-RFLP results, five<br> species including Malassezia globosa, Malassezia furfur, Malassezia restricta, Malassezia<br> sympodialis and Malassezia slofiae were identified.<br> Conclusion: Based on PCR-RFLP analysis in healthy control, seborrheic dermatitis and Pityriasis<br> versicolor in patients referred to laboratories in Isfahan, Malassezia globosa was the most common<br> isolated species among the five isolated species.Introduction: Malassezia spp. are lipophilic basidiomycetes that are the microbial members of the<br> flora of most warm-blooded animals. The natural habitat of these lipophilic yeasts is the horny part of<br> human and animal skin. Malassezia population densities in infected lesions are generally higher than<br> in healthy skin, and yeast proliferation appears to be the first step in developing Malassezia dermatitis.<br> Under certain conditions, these yeasts can cause diseases such as Pityriasis versicolor and seborrheic<br> dermatitis, and even cause systemic infections. The aim of this study was to investigate the prevalence<br> of Malassezia in Isfahan, Iran.<br> Material and methods: In this descriptive cross-sectional study in 2011 in Isfahan, 100 people (70<br> men and 30 women) with seborrheic dermatitis were studied. Sampling was performed by chipping<br> from dandruff and behind the ears. The samples were studied microscopically. After culturing and<br> extracting DNA from the colonies, Malassezia spp. were identified by PCR-RFLP method.<br> Results: Based on the results, the total number of yeast cells in the samples of patients with seborrheic<br> dermatitis was 53 (53%) and also 38 (38%) of yeast colonies grew. Based on PCR-RFLP results, five<br> species including Malassezia globosa, Malassezia furfur, Malassezia restricta, Malassezia<br> sympodialis and Malassezia slofiae were identified.<br> Conclusion: Based on PCR-RFLP analysis in healthy control, seborrheic dermatitis and Pityriasis<br> versicolor in patients referred to laboratories in Isfahan, Malassezia globosa was the most common<br> isolated species among the five isolated species. Malassezia spp, Pityriasis versicolor, Seborrheic dermatitis, MLNA culture medium, PCR-RFLP. 21 28 http://intjmi.com/browse.php?a_code=A-10-1-330&slc_lang=en&sid=1 Maryam Sarlak 10031947532846003801 10031947532846003801 No Department of Microbiology, Islamic Azad University, Falavarjan Branch, Isfahan, Iran. Pegah Shakib 10031947532846003802 10031947532846003802 No Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran. Mohammad Ali Zia 10031947532846003803 10031947532846003803 No Department of Basic Science, Islamic Azad University, Khorasgan Branch, Isfahan, Iran. Mahboobe Madani 10031947532846003804 10031947532846003804 Yes Department of Microbiology, Islamic Azad University, Falavarjan Branch, Isfahan, Iran.