Journal of Emergency Health Care

fa The Beneficial Effects Of Garlic And Mountain Tea Hydroalcoholic Extract On Methotrexate-Induced Liver Toxicity Through Attenuation Oxidative Stress عمومى General پژوهشي Research Background: Methotrexate (MTX), a chemotherapeutic and immunosuppressant drug, is generally well-tolerated by most patients. However, its cytotoxic nature contributes to life-threatening side effects including hepatotoxicity and nephrotoxicity. Several studies have already confirmed that the oxidative stress plays a major role in the pathogenesis of MTX-induced damage in the various organs. This study was carried out to determine whether garlic extract  has a protective effect against MTX-induced hepatotoxicity and nephrotoxicity. Materials and methods: 56 rats were divided randomly into six groups: Group I (control): treated with normal salin. Group II (MTX): was received sin-dose MTX(20<m:omath><m:f><m:fpr><m:type m:val="skw"><m:ctrlpr></m:ctrlpr></m:type></m:fpr><m:num><m:r>mg</m:r></m:num><m:den><m:r>kg</m:r></m:den></m:f></m:omath>i.p, 7th ). The third, fourth and fifth groups of the extract received 100, 250 and 500 mg/kg doses per day, respectively, and on day 7, methotrexate was given intraperitoneally at a dose of 20. Sixth, seventh and eighths of the group received the tea extract from 30, 50 and 70 mg/kg doses per day, respectively, and on day 7 received methotrexate intraperitoneally at a dose of 20 mg/kg. After 11 days, the liver tissue was  removed for measure the MDA, TAC, GSH and CAT, SOD activity in tissue homogeneity and histopathologic studies. Results: The results showed that CAT(P <0.001), TAC(P <0.01) and GSH (P <0.05)levels decreased in methotrexate group compared to control group. These values increased in the third to the eighth groups compared to the methotrexate group. Also, the MDA content in methotrexate group increased compared with the control group(P <0.001), which decreased in the third to eighth groups compared to the methotrexate group. MTX  had no significant effect on SOD levels. Conclusion:  Our results demonstrate that the prescription of garlic extract and mountain tea has a protective role against methotrexate's liver toxicity, which can be due to their antioxidant properties.   Methotrexate, garlic extract, mountain tea extract, liver, Oxidative stress, Histopathology 0 0 http://intjmi.com/browse.php?a_code=A-10-1-703&slc_lang=fa&sid=1 Sahar Behvandi 10031947532846008453 0009-0008-4077-0735 Yes Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran. Mohammad Mohammadi 10031947532846008454 10031947532846008454 No Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran Babak Mohammadian 10031947532846008455 10031947532846008455 No Department of Basic science, Faculty of Veterinary Medicine , Shahid Chamran University of Ahvaz, Ahvaz, Iran