[Home ] [Archive]    
:: Main About journal Editorial Board Current Issue Archive Submit an article Site Map Contact ::
:: Volume 10, Issue 4 (12-2021) ::
Int J Med Invest 2021, 10(4): 164-172 Back to browse issues page
Histological Effect Of Hydroalcoholic Extract Of Clover On Oxidative Stress And The Formation Of Ovarian Follicles In Wistar Rats
Ali Akbar Amiri, Hanieh Bagheri * , Mahdieh Raeiszadeh
Faculty of Veterinary Medicine, Islamic Azad University of Sanandaj, Sanandajm Iran.
Abstract:   (617 Views)
Introduction: Nowadays, infertility problems such as lack of follicle formation with various folliclestimulating compounds, antiestrogenic compounds such as clomiphene, tamoxifen, and gonadotropins such as FSH and LH have been treated. The aim of this study was the histological effect of hydroalcoholic clover extract on oxidative stress and the formation of ovarian follicles in female Wistar rats. Methods: In this study, 24 adult Wistar female rats were divided into 4 separate groups (control group, hydroalcoholic extract of clover 25, 50, and 100 mg/kg intraperitoneally for 25 days). On the last day, tissue samples were evaluated by vaginal smear and Giemsa staining. the left ovary was used for pathological sections and hematoxylin-eosin staining for follicle counting. The right ovary was used to measure Methylenedioxyamphetamine (MDA) levels. Ferric Reducing Ability of Plasma was used to determine the total antioxidant capacity (TAC) of serum. Results: A group receiving 100 mg/kg clover had higher mean ovarian weight, total antioxidant capacity, LH, FSH, Estradiol, and progesterone concentrations, and lower MDA compared to control and some other doses of clover (P<0.05). but, follicles count did not differ among study groups, except for atretic folliclesthat were significantly lower in the group receiving 100 mg/kg clover in comparison to control (P=0.001). Conclusion: This study shows that clover could significantly justify oxidative stress, inhibiting the breakdown of the ovarian follicles to atretic follicles, and can modify hormonal responses of the rat body in a dose-dependent manner.
Keywords: Hydroalcoholic extract of clover, Oxidative stress, Ovarian follicles
Full-Text [PDF 270 kb]   (163 Downloads)    
Type of Study: Research | Subject: General
1. 1. Aflatoonian A, Seyedhassani SM, Tabibnejad N. The epidemiological and etiological aspects of ‎Infertility in Yazd province of Iran. Jnt J Reprod Bio Med. 2009 Summer;7(3):117-122.‎ 2. Duleba AJ. Medical management of metabolic dysfunction in pcos. Steroids 2012; 77(4): 306-11.‎ 3. Ruder EH, Hartman TJ, Blumberg J & Goldman M. Oxidative stress and antioxidants: Exposure ‎and impact on female fertility. Hum Reprod Update 2008; 14(4): 345-57.‎ 4. Liu J, Liu M, Ye X, Liu K, Huang J, Wang L, et al. Delay in oocyte aging in mice by the ‎antioxidant n-acetyl-l-cysteine (nac). Human Reproduction 2012; 27(5): 1411-20.‎ 5. Herbison AE. Physiology of the gonadotropin-releasing hormone neuronal network. In: Neill JD, ‎Plant TM, editors. Knobil and Neil’s Physiology of Reproduction, 3rd ed. NewYork: Raven ‎Press, 2006:1415–1482.‎ 6. Ahmad N, Rahman Z, Akhtar N et al. Effect of madicago sativa on some serum biochemical ‎metabolites in rats. Int J AgricBiol. 2013; 15(2):297-300 ‎ 7. Monsefi, M., Masoudi, M., Hosseini, E., Gramifar, F., and Miri, R., 2012. Anti-fertility effects of ‎different fractions of Anethum graveolens L. extracts on female rats. African Journal ‎Traditional, Complementary, and Alternative Medicine, 9(3), PP:336-341‎ 8. Ososki, A.L., and Kennelly, E.J., 2003. Phytoestrogens: a review of the present state of research. ‎Phytotherapy Research, 17, PP: 845-869.‎ 9. Zhao, E., and Qing, M.U., 2010. Phytoestrogen Biological Actions on Mammalian Reproductive ‎System and Cancer Growth. Scientia Pharmaceutica, 79(1), PP: 1-20.‎ 10. Tundis R, Marrelli M, Conforti F, Tenuta M, Bonesi M, Menichini F, Loizzo M. Trifolium pratense ‎and T. repens (Leguminosae): Edible flower extracts as functional ingredients. Foods. 2015 ‎‎;4(3):338-48. ‎ 11. Schütz K, Carle R, Schieber A. Taraxacum—a review on its phytochemical and pharmacological ‎profile. Journal of ethnopharmacology. 2006;107(3): 313-23. ‎ 12. Azad Bakht M. Phytoestrogens. Journal of Medical Plants. 2006; 6(21):1–10‎ 13. Muhammad N, Saeed M, Khan H. Antipyretic, analgesic and antiinflammatory activity of Viola ‎betonicifolia whole plant. BMC complementary and alternative medicine. 2012 ;12(1):59.‎ 14. Abasian, Z., Rostamzadeh, A., Mohammadi, M., Hosseini, M., & Rafieian-Kopaei, M. (2018). A ‎review on role of medicinal plants in polycystic ovarian syndrome: Pathophysiology, ‎neuroendocrine signaling, therapeutic status and future prospects. Middle East Fertility Society ‎Journal, 23(4), 255-262.‏ 15. Abbasian, Z., Barmak, M. J., Barazesh, F., Ghavamizadeh, M., & Mirzaei, A. (2020). Therapeutic ‎efficacy of Trifolium pratense L. on letrozole induced polycystic ovary syndrome in rats. Plant ‎Science Today, 7(3), 501-507.‏ 16. Kim, M. R., Kim, H. J., Yu, S. H., Lee, B. S., Jeon, S. Y., Lee, J. J., & Lee, Y. C. (2020). Combination of red ‎clover and hops extract improved menopause symptoms in an ovariectomized rat ‎model. Evidence-Based Complementary and Alternative Medicine, 2020.‏ 17. Parandin, R., & Mohammadi, L. (2019). The effects of Formononetin Derived from Red Clover During ‎Pregnancy on Puberty, Some Reproductive Parameters and Lordosis Behavior of Female ‎Mice. Armaghane danesh, 24(2), 199-213.‏ 18. ABBASIAN Z, GHANBARI A. HYDROALCOHOLIC EXTRACT OF RED CLOVER (TRIFOLIUM PRETENSE) ‎IMPROVES HORMONAL BALANCES AFTER INDUCTION OF POLYCYSTIC OVARY SYNDROME IN ‎RATS.‎ 19. Kim, H. S., Shin, J. H., Moon, H. J., Kim, T. S., Kang, I. H., Seok, J. H., ... & Han, S. Y. (2002). Evaluation ‎of the 20-day pubertal female assay in Sprague-Dawley rats treated with DES, tamoxifen, ‎testosterone, and flutamide. Toxicological Sciences, 67(1), 52-62.‏ 20. Chattopadhyay, S., Pal, S., Ghosh, D., & Debnath, J. (2003). Effect of dietary co-administration of ‎sodium selenite on sodium arsenite-induced ovarian and uterine disorders in mature albino ‎rats. Toxicological Sciences, 75(2), 412-422.‏ 21. Abdulghani, M., Hussin, A. H., Sulaiman, S. A., & Chan, K. L. (2012). The ameliorative effects of ‎Eurycoma longifolia Jack on testosterone-induced reproductive disorders in female rats. ‎Reproductive biology, 12(2), 247-255.‏ 22. Trisunuwati, P. (2017). The role of leaf water clover (Marsilia Crenata) squeeze towards estrogen ‎blood level and uterine histology in rats (Rattus norvegicus). TERNAK TROPIKA Journal of Tropical ‎Animal Production, 17(2), 1-7.‏ 23. Burdette, J. E., Liu, J., Lantvit, D., Lim, E., Booth, N., Bhat, K. P., ... & Bolton, J. L. (2002). Trifolium ‎pratense (red clover) exhibits estrogenic effects in vivo in ovariectomized Sprague-Dawley ‎rats. The Journal of nutrition, 132(1), 27-30.‎ 24. Widhaningrum, S. N., Rahayu, S., & Ciptadi, G. (2021). Effect of Water Clover (Marsilea crenata) ‎Ethanol Extracts on Follicle and Oocyte Diameter of Goat: In Vitro Study. The Journal of ‎Experimental Life Science, 10(2), 113-118.‏
Add your comments about this article
Your username or Email:


XML     Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Amiri A A, Bagheri H, Raeiszadeh M. Histological Effect Of Hydroalcoholic Extract Of Clover On Oxidative Stress And The Formation Of Ovarian Follicles In Wistar Rats. Int J Med Invest. 2021; 10 (4) :164-172
URL: http://intjmi.com/article-1-723-en.html

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 10, Issue 4 (12-2021) Back to browse issues page
International Journal of Medical Investigation
Persian site map - English site map - Created in 0.04 seconds with 29 queries by YEKTAWEB 4410