International Journal of Medical Investigation
Int J Med Invest
Medical Sciences
http://intjmi.com
1
admin
2322-2913
2345-640x
no
no
14
8888
13
en
jalali
1396
6
1
gregorian
2017
9
1
6
3
online
1
fulltext
en
Comparison of PCR and culture methods for detecting of Listeria monocytogenes in vaginal samples
عمومى
General
پژوهشي
Research
Listeria monocytogenes is an opportunistic intracellular pathogen that has become an important cause of human food borne infections worldwide. The aim of this study is the determinate sensitivity of diagnostic methods of this organism and achieving a test that can detect Listeria monocytogenes in all circumstances in clinical samples. Materials and Methods: In this study 88 vaginal swabs were collected from women with age of (15-45) referred to gynecologic clinics in the hospital (Sari, Iran). Vaginal swabs were examined by culture on specific PALKAM agar medium and PCR technique by specific primers. Results: Out of 88 studied vaginal swabs, 8 cases with culture method and 27 cases were positive with PCR technique. The sensitivity of PCR and culture is reported %100, %29 respectively. Conclusion: The results show that PCR is a more sensitive, easier, and faster method in comparison to in clinical samples.
Listeria monocytogenes, PCR, culture methods
71
74
http://intjmi.com/browse.php?a_code=A-10-1-175&slc_lang=en&sid=1
Mohtaram
Nasrollahi
10031947532846002799
10031947532846002799
No
Professor in Microbiology, Department of Microbiology, Mazandaran University of Medical Science, Mazandaran, Sari, Iran.
Zeynab
Marzhoseyni
10031947532846002800
10031947532846002800
No
MSc in Microbiology, Department of Microbiology, Mazandaran University of Medical Science, Mazandaran, Sari, Iran
Tahoora
Mousavi
10031947532846002801
10031947532846002801
No
Ph.D. Student in Molecular and Cell Biology, Student Research Committee, Molecular and Cell Biology Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
Mohammad
Ahanjan
10031947532846002802
10031947532846002802
Yes
Associate Professor, Department of Microbiology, Mazandaran University of Medical Science, Mazandaran, Sari, Iran.