International Journal of Medical Investigation
Volume 9, Issue 4 (12-2020)
Int J Med Invest 2020, 9(4): 21-28 |
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Sarlak M, Shakib P, Zia M A, Madani M. The Prevalence of Malassezia yeasts in patients with seborrheic dermatitis by
PCR-RFLP method in Isfahan, Iran. Int J Med Invest 2020; 9 (4) :21-28
URL: http://intjmi.com/article-1-560-en.html
URL: http://intjmi.com/article-1-560-en.html
Department of Microbiology, Islamic Azad University, Falavarjan Branch, Isfahan, Iran.
Abstract: (3000 Views)
Introduction: Malassezia spp. are lipophilic basidiomycetes that are the microbial members of the
flora of most warm-blooded animals. The natural habitat of these lipophilic yeasts is the horny part of
human and animal skin. Malassezia population densities in infected lesions are generally higher than
in healthy skin, and yeast proliferation appears to be the first step in developing Malassezia dermatitis.
Under certain conditions, these yeasts can cause diseases such as Pityriasis versicolor and seborrheic
dermatitis, and even cause systemic infections. The aim of this study was to investigate the prevalence
of Malassezia in Isfahan, Iran.
Material and methods: In this descriptive cross-sectional study in 2011 in Isfahan, 100 people (70
men and 30 women) with seborrheic dermatitis were studied. Sampling was performed by chipping
from dandruff and behind the ears. The samples were studied microscopically. After culturing and
extracting DNA from the colonies, Malassezia spp. were identified by PCR-RFLP method.
Results: Based on the results, the total number of yeast cells in the samples of patients with seborrheic
dermatitis was 53 (53%) and also 38 (38%) of yeast colonies grew. Based on PCR-RFLP results, five
species including Malassezia globosa, Malassezia furfur, Malassezia restricta, Malassezia
sympodialis and Malassezia slofiae were identified.
Conclusion: Based on PCR-RFLP analysis in healthy control, seborrheic dermatitis and Pityriasis
versicolor in patients referred to laboratories in Isfahan, Malassezia globosa was the most common
isolated species among the five isolated species.Introduction: Malassezia spp. are lipophilic basidiomycetes that are the microbial members of the
flora of most warm-blooded animals. The natural habitat of these lipophilic yeasts is the horny part of
human and animal skin. Malassezia population densities in infected lesions are generally higher than
in healthy skin, and yeast proliferation appears to be the first step in developing Malassezia dermatitis.
Under certain conditions, these yeasts can cause diseases such as Pityriasis versicolor and seborrheic
dermatitis, and even cause systemic infections. The aim of this study was to investigate the prevalence
of Malassezia in Isfahan, Iran.
Material and methods: In this descriptive cross-sectional study in 2011 in Isfahan, 100 people (70
men and 30 women) with seborrheic dermatitis were studied. Sampling was performed by chipping
from dandruff and behind the ears. The samples were studied microscopically. After culturing and
extracting DNA from the colonies, Malassezia spp. were identified by PCR-RFLP method.
Results: Based on the results, the total number of yeast cells in the samples of patients with seborrheic
dermatitis was 53 (53%) and also 38 (38%) of yeast colonies grew. Based on PCR-RFLP results, five
species including Malassezia globosa, Malassezia furfur, Malassezia restricta, Malassezia
sympodialis and Malassezia slofiae were identified.
Conclusion: Based on PCR-RFLP analysis in healthy control, seborrheic dermatitis and Pityriasis
versicolor in patients referred to laboratories in Isfahan, Malassezia globosa was the most common
isolated species among the five isolated species.
flora of most warm-blooded animals. The natural habitat of these lipophilic yeasts is the horny part of
human and animal skin. Malassezia population densities in infected lesions are generally higher than
in healthy skin, and yeast proliferation appears to be the first step in developing Malassezia dermatitis.
Under certain conditions, these yeasts can cause diseases such as Pityriasis versicolor and seborrheic
dermatitis, and even cause systemic infections. The aim of this study was to investigate the prevalence
of Malassezia in Isfahan, Iran.
Material and methods: In this descriptive cross-sectional study in 2011 in Isfahan, 100 people (70
men and 30 women) with seborrheic dermatitis were studied. Sampling was performed by chipping
from dandruff and behind the ears. The samples were studied microscopically. After culturing and
extracting DNA from the colonies, Malassezia spp. were identified by PCR-RFLP method.
Results: Based on the results, the total number of yeast cells in the samples of patients with seborrheic
dermatitis was 53 (53%) and also 38 (38%) of yeast colonies grew. Based on PCR-RFLP results, five
species including Malassezia globosa, Malassezia furfur, Malassezia restricta, Malassezia
sympodialis and Malassezia slofiae were identified.
Conclusion: Based on PCR-RFLP analysis in healthy control, seborrheic dermatitis and Pityriasis
versicolor in patients referred to laboratories in Isfahan, Malassezia globosa was the most common
isolated species among the five isolated species.Introduction: Malassezia spp. are lipophilic basidiomycetes that are the microbial members of the
flora of most warm-blooded animals. The natural habitat of these lipophilic yeasts is the horny part of
human and animal skin. Malassezia population densities in infected lesions are generally higher than
in healthy skin, and yeast proliferation appears to be the first step in developing Malassezia dermatitis.
Under certain conditions, these yeasts can cause diseases such as Pityriasis versicolor and seborrheic
dermatitis, and even cause systemic infections. The aim of this study was to investigate the prevalence
of Malassezia in Isfahan, Iran.
Material and methods: In this descriptive cross-sectional study in 2011 in Isfahan, 100 people (70
men and 30 women) with seborrheic dermatitis were studied. Sampling was performed by chipping
from dandruff and behind the ears. The samples were studied microscopically. After culturing and
extracting DNA from the colonies, Malassezia spp. were identified by PCR-RFLP method.
Results: Based on the results, the total number of yeast cells in the samples of patients with seborrheic
dermatitis was 53 (53%) and also 38 (38%) of yeast colonies grew. Based on PCR-RFLP results, five
species including Malassezia globosa, Malassezia furfur, Malassezia restricta, Malassezia
sympodialis and Malassezia slofiae were identified.
Conclusion: Based on PCR-RFLP analysis in healthy control, seborrheic dermatitis and Pityriasis
versicolor in patients referred to laboratories in Isfahan, Malassezia globosa was the most common
isolated species among the five isolated species.
Keywords: Malassezia spp, Pityriasis versicolor, Seborrheic dermatitis, MLNA culture medium, PCR-RFLP.
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