Journal of Emergency Health Care
Formerly known as: International Journal of Medical Investigation
Volume 11, Issue 4 (12-2022)
J Emerg Health Care 2022, 11(4): 147-158 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Laki M S, Hosseini F, Tabatabaei R R. The Effect Of Vancomycin-Loaded Niosomes On MRSA Strains Of Staphylococcus Aureus And Expression of MECA, HLA, and HLB Genes. J Emerg Health Care 2022; 11 (4) :147-158
URL: http://intjmi.com/article-1-938-en.html
URL: http://intjmi.com/article-1-938-en.html
Department of Microbiology, Islamic Azad University, Tehran North Branch, Tehran, Iran
Abstract: (1492 Views)
Background:
Niosomes have received a lot of attention today due to their better penetration and controlled release. This study aimed to evaluate the antimicrobial effect of vancomycin-loaded niosomes on the microbial species of staphylococcus aureus.
Materials and methods:
In this study, 250 clinical samples of different patients’ specimens including blood, wounds, skin, and urine were collected from different medical centers (Imam Hossein, Atieh, and Sarem) in 2020. To synthesize nanodrugs, vancomycin was encapsulated in nosocomial nanocarriers by thin-film hydration, and optimization was performed based on the three main characteristics of nanocarriers. The process of drug release from nanocarriers and stability were investigated. Then, the antimicrobial properties against microbial species of staphylococcus aureus were investigated, and finally, the expression of mecA, hla, and hlb genes was determined using the real-time PCR technique.
Results:
According to the designed tests, a Span60 to Tween60 ratio of 50:50 and lipid content of 300 μmol were selected as the optimal form. The optimal nanoliposomes showed a size of 190.7 nm, a particle dispersion index (PDI) of 0.177, and retention efficiency of 71.22%. The process of drug release from the nanocarrier showed about 50% drug release from the niosome during 24 hours and about 60% after 72 hours. Stability studies over 3 months at two temperatures of 25 °C and 4 °C on the optimal sample showed that the samples stored in the refrigerator were more stable. The antimicrobial properties of the vancomycin-loaded niosomes against the mentioned microbial species showed better results compared to the free form of the drug. The nanoparticle was also able to further reduce the expression of virulence genes including mecA, hla, and hlb compared to the free form of the drug.
Conclusion:
The favorable physical properties, efficient antimicrobial effects, and low toxicity make vancomycin-loaded niosome nanocarriers a suitable candidate for the treatment of some common bacterial wound infections.
Niosomes have received a lot of attention today due to their better penetration and controlled release. This study aimed to evaluate the antimicrobial effect of vancomycin-loaded niosomes on the microbial species of staphylococcus aureus.
Materials and methods:
In this study, 250 clinical samples of different patients’ specimens including blood, wounds, skin, and urine were collected from different medical centers (Imam Hossein, Atieh, and Sarem) in 2020. To synthesize nanodrugs, vancomycin was encapsulated in nosocomial nanocarriers by thin-film hydration, and optimization was performed based on the three main characteristics of nanocarriers. The process of drug release from nanocarriers and stability were investigated. Then, the antimicrobial properties against microbial species of staphylococcus aureus were investigated, and finally, the expression of mecA, hla, and hlb genes was determined using the real-time PCR technique.
Results:
According to the designed tests, a Span60 to Tween60 ratio of 50:50 and lipid content of 300 μmol were selected as the optimal form. The optimal nanoliposomes showed a size of 190.7 nm, a particle dispersion index (PDI) of 0.177, and retention efficiency of 71.22%. The process of drug release from the nanocarrier showed about 50% drug release from the niosome during 24 hours and about 60% after 72 hours. Stability studies over 3 months at two temperatures of 25 °C and 4 °C on the optimal sample showed that the samples stored in the refrigerator were more stable. The antimicrobial properties of the vancomycin-loaded niosomes against the mentioned microbial species showed better results compared to the free form of the drug. The nanoparticle was also able to further reduce the expression of virulence genes including mecA, hla, and hlb compared to the free form of the drug.
Conclusion:
The favorable physical properties, efficient antimicrobial effects, and low toxicity make vancomycin-loaded niosome nanocarriers a suitable candidate for the treatment of some common bacterial wound infections.
References
1. 1. Ryan KJ RC, eds. Sherris Medical Microbiology (4th ed.). McGraw Hill. 2004;ISBN 0-8385-8529-9.
2. LINDBOM G, LAURELL G, GRENVIK Å. Studies on the epidemiology of staphylococcal infections. APMIS. 1967;69(2):219-36.
3. Shinefield HR, Ruff NL. Staphylococcal infections: a historical perspective. Infectious disease clinics of North America. 2009;23(1):1-15.
4. Marsot A, Boulamery A, Bruguerolle B, Simon N. Vancomycin. Clinical pharmacokinetics. 2012;51(1):1-13.
5. Kazi KM, Mandal AS, Biswas N, Guha A, Chatterjee S, Behera M, et al. Niosome: a future of targeted drug delivery systems. Journal of advanced pharmaceutical technology & research. 2010;1(4):374.
6. Barani M, Mirzaei M, Torkzadeh-Mahani M, Nematollahi MH. Lawsone-loaded Niosome and its antitumor activity in MCF-7 breast Cancer cell line: a Nano-herbal treatment for Cancer. DARU Journal of Pharmaceutical Sciences. 2018;26(1):11-7.
7. Askari M, Nikoonahad Lotfabadi N. Evaluation of niosomal nano-carriers capabilities on toxicity preservation and delivery of pomegranate peel extract in cell culture conditions (MCF-7 cell line of breast cancer). Daneshvar Medicine. 2020;26(5):9-20.
8. Paolino D, Muzzalupo R, Ricciardi A, Celia C, Picci N, Fresta M. In vitro and in vivo evaluation of Bola-surfactant containing niosomes for transdermal delivery. Biomedical microdevices. 2007;9(4):421-33.
9. Asgharkhani E, Azarbayjani AF, Irani S, Chiani M, Saffari Z, Norouzian D, et al. Artemisinin-loaded niosome and pegylated niosome: physico-chemical characterization and effects on MCF-7 cell proliferation. Journal of Pharmaceutical Investigation. 2018;48(3):251-6.
10. Nikoonahad Lotfabadi N, Mohseni Kouchesfehani H, Sheikhha MH, Kalantar SM. Evaluation and comparison of physicochemical properties, cytotoxicity and the ability of miRNA loading of different cationic liposomes for gene therapy application. SSU_Journals. 2017;25(6):444-56.
11. Parthasarathi G, Udupa N, Umadevi P, Pillai G. Niosome encapsulated of vincristine sulfate: improved anticancer activity with reduced toxicity in mice. Journal of drug targeting. 1994;2(2):173-82.
12. Nikoonahad Lotfabadi N, Mohseni Kouchesfehani H, Sheikhha MH, Kalantar SM. MiRNA-101 transfection and its effect on the cytotoxicity induction and expression of ubiquitin ligase HECTH9 in acute myeloid leukemia cells(AML). SSU_Journals. 2018;26(1):64-76.
13. Mujoriya RZ, Dhamande K, Bodla R. Niosomal drug delivery system—a review. Int J Appl Pharm. 2011;3(3):7-10.
14. Amoabediny G, Haghiralsadat F, Naderinezhad S, Helder MN, Akhoundi Kharanaghi E, Mohammadnejad Arough J, et al. Overview of preparation methods of polymeric and lipid-based (niosome, solid lipid, liposome) nanoparticles: A comprehensive review. International Journal of Polymeric Materials and Polymeric Biomaterials. 2018;67(6):383-400.
15. Khan R, Irchhaiya R. Niosomes: a potential tool for novel drug delivery. Journal of pharmaceutical investigation. 2016;46(3):195-204.
16. Abdelaziz AA, Elbanna TE, Sonbol FI, Gamaleldin NM, El Maghraby GM. Optimization of niosomes for enhanced antibacterial activity and reduced bacterial resistance: in vitro and in vivo evaluation. Expert opinion on drug delivery. 2015;12(2):163-80.
17. Kashef MT, Saleh NM, Assar NH, Ramadan MA. The antimicrobial activity of ciprofloxacin-loaded niosomes against ciprofloxacin-resistant and biofilm-forming staphylococcus aureus. Infection and Drug Resistance. 2020;13:1619.
18. Davies J, Davies D. Origins and evolution of antibiotic resistance. Microbiology and molecular biology reviews. 2010;74(3):417-33.
19. Baillie A, Florence A, Hume L, Muirhead G, Rogerson A. The preparation and properties of niosomes—non‐ionic surfactant vesicles. Journal of pharmacy and pharmacology. 1985;37(12):863-8.
20. Ge X, Wei M, He S, Yuan W-E. Advances of non-ionic surfactant vesicles (niosomes) and their application in drug delivery. Pharmaceutics. 2019;11(2):55.
21. Bini K, Akhilesh D, Prabhakara P, Kamath J. Development and characterization of non-ionic surfactant vesicles (niosomes) for oral delivery of lornoxicam. International Journal of Drug Development and Research. 2012;4(3):147-54.
22. Taymouri S, Varshosaz J. Effect of different types of surfactants on the physical properties and stability of carvedilol nano-niosomes. Advanced biomedical research. 2016;5.
23. Patra JK, Das G, Fraceto LF, Campos EVR, del Pilar Rodriguez-Torres M, Acosta-Torres LS, et al. Nano based drug delivery systems: recent developments and future prospects. Journal of nanobiotechnology. 2018;16(1):1-33.
24. Kamboj S, Saini V, Bala S, Sharma G. Formulation and characterization of drug loaded niosomal gel for anti-inflammatory activity. International Journal of Pharmacological and Pharmaceutical Sciences. 2013;7(12):877-81.
25. Akbarzadeh I, Yaraki MT, Ahmadi S, Chiani M, Nourouzian D. Folic acid-functionalized niosomal nanoparticles for selective dual-drug delivery into breast cancer cells: An in-vitro investigation. Advanced Powder Technology. 2020;31(9):4064-71.
26. Haeri A, Sadeghian S, Rabbani S, Shirani S, Anvari MS, Dadashzadeh S. Physicochemical characteristics of liposomes are decisive for their antirestenosis efficacy following local delivery. Nanomedicine. 2017;12(2):131-45.
27. Ghafelehbashi R, Akbarzadeh I, Yaraki MT, Lajevardi A, Fatemizadeh M, Saremi LH. Preparation, physicochemical properties, in vitro evaluation and release behavior of cephalexin-loaded niosomes. International journal of pharmaceutics. 2019;569:118580.
28. Heidari F, Akbarzadeh I, Nourouzian D, Mirzaie A, Bakhshandeh H. Optimization and characterization of tannic acid loaded niosomes for enhanced antibacterial and anti-biofilm activities. Advanced Powder Technology. 2020;31(12):4768-81.
29. Mirzaie A, Peirovi N, Akbarzadeh I, Moghtaderi M, Heidari F, Yeganeh FE, et al. Preparation and optimization of ciprofloxacin encapsulated niosomes: A new approach for enhanced antibacterial activity, biofilm inhibition and reduced antibiotic resistance in ciprofloxacin-resistant methicillin-resistance Staphylococcus aureus. Bioorganic Chemistry. 2020;103:104231.
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |