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:: Volume 8, Issue 2 (5-2019) ::
Int J Med Invest 2019, 8(2): 1-5 Back to browse issues page
Technical Points in Vitamin D Measurement Assays
Mehri Aliasgharpour *
Ministry of Health & Medical Education, Reference Health Laboratory, Biochemistry Laboratory
Abstract:   (1825 Views)
Background and aim: Major sources for vitamin D (Vit D3& Vit D2) in humans are skin and diet. Vitamin D is needed to maintain calcium concentrations within a narrow physiologic range and its status is assessed by measuring total serum levels of 25-hydroxyvitamin D, 25(OH) D metabolite. Despite the availability of many methods including High Performance Liquid Chromatography (HPLC), Liquid Chromatography -Mass Spectrometry (LC-MS/MS) and different immunoassays, the sensitivities and specifications of these vary considerably.
Methods: For the present paper, electronic databases including; PubMed, Scopus, Scientific Information Database, and etc during years 2000 to 2019 were selected and related papers were reviewed.
Results: The investigations indicated significant laboratory variation on the values generated for the 25(OH) D metabolite measurement in the same specimen using different methods and assays.
Conclusion: In summary, the choice to which assay or method to use for routine serum vitamin D determination will depend on the available equipment and expertise of each laboratory. In addition, to the contribution of different international programs for vitamin D metabolites measurement- to minimize inter assay and intra assay laboratory variations- it is suggested to use a suitable commercial serum control materials along with the manufacture’s quality control material for vitamin D routine determinations. So that each laboratory can establish its own assay specific clinical decision limits based on the institute of medicine recommendations for low and upper safe limits of vitamin D.
Keywords: Vitamin D- Immunoassay- Accuracy- Precision- Sensitivity - Specificity
Full-Text [PDF 133 kb]   (427 Downloads)    
Type of Study: Research | Subject: General
1. 1. Lips P, Van Schoor NM. The effect of vitamin D on bone and osteoporosis. Best Pract. Res. Clin. Endocrinol. Metab 2011; 25 (4), 585–91 2. Norman AW. From vitamin D to hormone D: fundamentals of the vitamin D endocrine system essential for good health. Am J Clin Nutr. 2008;88(2):491S-499S. 3. Galior K, Ketha H, Grebe S, Singh RJ. 10 years of 25-hydroxyvitamin-D testing by LC-MS/MS-trends in vitamin-D deficiency and sufficiency. Bone Rep 2018; 8: 268–273. 4. Holick M.F. Deficiency of sunlight and vitamin D. BMJ. 2008;336(7657):1318–1319. 5. Gilchrest BA. Sun protection and vitamin D: three dimensions of obfuscation. J Steroid Biochem Mol Biol 2007;103:655– 63. 6. Standing Committee on the Scientific Evaluation of Dietary Reference Intakes, Food and Nutrition Board, Institute of Medicine Vitamin D. Dietary reference intakes for calcium, magnesium, phosphorus, vitamin D, and fluoride.Washington, DC National Academy Press1997. 7. Pike JW, Meyer MB, Benkusky NA, Lee SM, St John H, Carlson A, Onal M, Shamsuzzaman S.Genomic determinants of vitamin D-regulated gene expression. Vitam. Horm 2016;100:21–44. 8. Haussler MR. Vitamin D receptors: nature and function. Ann. Revs. Nutr 1986; 6:527-562 9. Kumar J, Yadav, A, Agrawal, K. (2018) Vitamin D deficiency: An emerging pandemic. J Family Med Prim Care 2018; 7(5): 1146–1147. 10. Bischoff-Ferrari HA, Dietrich T, Orav EJ, Dawson-Hughes B. Positive association between 25-hydroxy vitamin D levels and bone mineral density: a population-based study of younger and older adults. Am J Med 2004;116(9): 634–639. 11. Bouillon R, Suda, T,. (2014) Vitamin D: calcium and bone homeostasis during evolution. Bone key Rep 2014; 3:480. 12. Lips P. Vitamin D deficiency and secondary hyperparathyroidism in the elderly: consequences for bone loss and fractures and therapeutic implications. Endocr Rev 2001; 22: 477–501. 13. Bischoff-Ferrari HA, Dietrich T, Orav EJ, Hu FB, Zhang Y, et al. Higher 25-hydroxyvitamin D concentrations are associated with better lower-extremity function in both active and inactive persons aged > or = 60 y. Am J Clin Nutr 2004;80(3): 752-8. 14. Holick MF. Vitamin D: importance in the prevention of cancers, type 1 diabetes, heart disease, and osteoporosis. Am J Clin Nutr 2004; 79: 362–371. 15. Forman JP, Bischoff-Ferrari HA, Willett WC, Stampfer MJ, Curhan GC .Vitamin D intake and risk of incident hypertension: results from three large prospective cohort studies. Hypertension 2005; 46: 676–682. 16. Pludowski, P, Holick M.F, Pilz S. et al. Vitamin D effects on musculoskeletal health, immunity, autoimmunity, cardiovascular disease, cancer, fertility, pregnancy, dementia and mortality‐a review of recent evidence, Autoimmun Rev 2013; 12: 976–989. 17. Gonzalez Nguyen-Tang E, Parvex P, Goischke A, Wilhelm-Bals A. Vitamin D deficiency and rickets : screening and treatment, practical aspects for clinicians] . Rev Med Suisse. 2019;15(638):384-389. 18. Melamed ML, Michos ED, Post W, Astor B .25-hydroxyvitamin D levels and the risk of mortality in the general population. Arch Intern Med 2008;168: 1629–1637. 19. Singh RJ. Are clinical laboratories prepared for accurate testing of 25-hydroxy vitamin D? Clin. Chem 2008;54(1):221–223. 20. Snellman G, Melhus H, Gedeborg R., Byberg L. et al. Determining vitamin D Status: A comparison between commercially available assays. PloS ONE. 2010;5(7):e11555. 21. Farrell CJ, Martin S, McWhinney B. et al. State of the art vitamin D assays; a comparison of automated immunoassays with liquid chromatography-tandem mass spectrometry methods, Clin Chem 2012; 58;531-542. 22. Heijboer AC, Blankenstein MA, Kema IP, Buijs MM. Accuracy of 6 routine 25-hydroxyvitamin D assays: influence of vitamin D binding protein concentration. Clin. Chem 2012;58(3):543–548. 23. Grebe S., Singh RJ. LC-MS/MS in the clinical laboratory - where to from here? Clin. Biochem. Rev 2011; 32 (1), 5–31. 24. Burdette CQ, Camara J, Nalin, F, Pritchett J, Sander LC, Arid GDC. et al. Establishing an accuracy basis for the vitamin D external quality assessment scheme (DEQAS). J. AOAC Int 2017; 100 (5), 1277–1287. 25. Sempos, CT, Vesper HW, Phinney KW. et al. Vitamin D Standardization program(VDSP): Vitamin D status as an international issue: National surveys and the problem of standardization . Scand J Clin Lab Invest Suppl 2012;243:32-40. 26. Wise SA, Phinney KW, Tai SS, Camara JE, Myers GL, Durazo-Arvizu R. et al. Baseline assessment of 25-hydroxyvitamin D assayperformance: a vitamin D standardization program (VDSP) inter laboratory comparison study. J. AOAC Int 2017; 100(5), 1244–1252. 27. Carter GD. Accuracy of 25 hydroxyvitamin D assays: confronting the issues. Curr Drug Targets 2011; 12:19-28. 28. Glenville J. Interpreting vitamin D assay results: proceed with caution. Clin J am Soc Nephrol 2015;10(2):331-334. 29. Binkley N, Krueger DC, Morgan S, Wiebe D. Current status of clinical 25-hydroxyvitamin D measurement: an assessment of between laboratory agreement, Clin Chem Acta,2010; 411:1976-1982. 30. Ross AC, Manson JE, Abrams SA. et al. The 2011 report on dietary reference intakes for calcium and vitamin D from the Institute of Medicine: what clinicians need to know, J Clin Endocrinol Metab 2011; 96:53-58.
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Aliasgharpour M. Technical Points in Vitamin D Measurement Assays. Int J Med Invest. 2019; 8 (2) :1-5
URL: http://intjmi.com/article-1-393-en.html

Volume 8, Issue 2 (5-2019) Back to browse issues page
International Journal of Medical Investigation
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