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:: Volume 7, Issue 2 (6-2018) ::
Int J Med Invest 2018, 7(2): 16-20 Back to browse issues page
Ethylene Glycol Induced Calcium Oxalate Crystals and Oxidative Damage of Renal cells
Mohammadreza Ebadzadeh , Sharyar Dabiri , Mohammad Khaksari , Seyed Jamiladin Fatemi , Rayka Sharifian * , Fetemeh Ghanbarpour , Nasrin Partovi
Resident of Urology, Kerman University of Medical Sciences, Kerman, Iran
Abstract:   (8240 Views)
Introduction: : Urolithiasis is one of common renal disease that attributed to multiple factors. In other hand, calcium oxalate is the most common urine lithiasis and urinary calcium and oxalate oversaturation are both important to calcium oxalate stone formation. The percent study was undertaken to examine the possible role of oxidative stress and cell injury in stone formation. Method: In this research 12 rats with average weight of (200-250) ±6 grams in two groups, accidentally were chosen. Stone group animals consume ethylene glycol 0.75% and ammonium chloride 1% for three days and then ethylene glycol 0.75% for 25 days to create stones in their kidneys. Control group rats maintained on regular food and drinking for 28 days. The status of oxidative stress induced by Calcium oxalate was evaluated by malondialdehyde and superoxide dismutase in renal tissue. Findings: In present study, the body weight gain was small in stone group compare control group but kidney weight increased significantly. Many crystals deposit of Caox were seen in all regions of renal tubules in stone group. Calcium excretion increased after stone formation but magnesium decreased significantly. Urinary creatinine content was higher in control group. The serum creatinine, urea and uric acid were remarkably increased in urolithiasis induced group compare with control group. Superoxide dismutase activity decreased significantly in stone groups but MDA production increased in stone group when compare with control group. Conclusion: Our results indicate that ethylene glycol produces oxidative stress in the kidneys as shown by increased tissue MDA significantly. The antioxidant agents like vitamins and etc. can reverse these defective cycles properly.
Keywords: Urolithiasis, Calcium Oxalate, Malondialdehyde, Superoxide Dismutase
Full-Text [PDF 538 kb]   (1271 Downloads)    
Type of Study: Research | Subject: General
References
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Ebadzadeh M, Dabiri S, Khaksari M, Fatemi S J, Sharifian R, Ghanbarpour F et al . Ethylene Glycol Induced Calcium Oxalate Crystals and Oxidative Damage of Renal cells. Int J Med Invest 2018; 7 (2) :16-20
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Volume 7, Issue 2 (6-2018) Back to browse issues page
International Journal of Medical Investigation
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