Histological Effect Of Hydroalcoholic Extract Of Clover On Oxidative Stress And The
Formation Of Ovarian Follicles In Wistar Rats

Amiri, Ali Akbar; Bagheri, Hanieh; Raeiszadeh, Mahdieh

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Volume 10, Issue 4 (12-2021)

Int J Med Invest 2021, 10(4): 164-172

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Histological Effect Of Hydroalcoholic Extract Of Clover On Oxidative Stress And The Formation Of Ovarian Follicles In Wistar Rats

Ali Akbar Amiri

, Hanieh Bagheri ^*

, Mahdieh Raeiszadeh

Faculty of Veterinary Medicine, Islamic Azad University of Sanandaj, Sanandajm Iran.

Abstract: (1978 Views)

Introduction: Nowadays, infertility problems such as lack of follicle formation with various folliclestimulating compounds, antiestrogenic compounds such as clomiphene, tamoxifen, and gonadotropins such as FSH and LH have been treated. The aim of this study was the histological effect of hydroalcoholic clover extract on oxidative stress and the formation of ovarian follicles in female Wistar rats. Methods: In this study, 24 adult Wistar female rats were divided into 4 separate groups (control group, hydroalcoholic extract of clover 25, 50, and 100 mg/kg intraperitoneally for 25 days). On the last day, tissue samples were evaluated by vaginal smear and Giemsa staining. the left ovary was used for pathological sections and hematoxylin-eosin staining for follicle counting. The right ovary was used to measure Methylenedioxyamphetamine (MDA) levels. Ferric Reducing Ability of Plasma was used to determine the total antioxidant capacity (TAC) of serum. Results: A group receiving 100 mg/kg clover had higher mean ovarian weight, total antioxidant capacity, LH, FSH, Estradiol, and progesterone concentrations, and lower MDA compared to control and some other doses of clover (P<0.05). but, follicles count did not differ among study groups, except for atretic folliclesthat were significantly lower in the group receiving 100 mg/kg clover in comparison to control (P=0.001). Conclusion: This study shows that clover could significantly justify oxidative stress, inhibiting the breakdown of the ovarian follicles to atretic follicles, and can modify hormonal responses of the rat body in a dose-dependent manner.

Keywords: Hydroalcoholic extract of clover, Oxidative stress, Ovarian follicles

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Type of Study: Research | Subject: General

References

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Amiri A A, Bagheri H, Raeiszadeh M. Histological Effect Of Hydroalcoholic Extract Of Clover On Oxidative Stress And The Formation Of Ovarian Follicles In Wistar Rats. Int J Med Invest 2021; 10 (4) :164-172
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