Journal of Emergency Health Care
Formerly known as: International Journal of Medical Investigation
Volume 11, Issue 4 (12-2022)
J Emerg Health Care 2022, 11(4): 109-114 |
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Maragheh S M, Zakariya A A, Ghaedi E, Saeifar F, Talemi M N, Varjovi M R et al . Evaluation of rs7903146 Polymorphism in TCF7L2 Gene among Type 2 Diabetes Patients. J Emerg Health Care 2022; 11 (4) :109-114
URL: http://intjmi.com/article-1-934-en.html
URL: http://intjmi.com/article-1-934-en.html
Salar Momen Maragheh 
, Alireza Aslani Zakariya , Elham Ghaedi , Fahimeh Saeifar , Mahdi Navid Talemi , Mehran Rezanezhad Varjovi , Mahdi Bagheri * 
, Alireza Aslani Zakariya , Elham Ghaedi , Fahimeh Saeifar , Mahdi Navid Talemi , Mehran Rezanezhad Varjovi , Mahdi Bagheri *
Department of Medicine, Medical University of Ilam, Ilam, Iran
Abstract: (4014 Views)
Background:
Diabetes is characterized by decreased insulin sensitivity and is a growing health threat worldwide. Therefore, this study aimed to evaluate the relationship between rs7903146 polymorphism in the TCF7L2 gene with type 2 diabetes in East Azerbaijan province, Iran.
Materials and method:
A total of 100 blood samples were collected from diabetic and healthy people. The samples were subjected to specific primers, PCR, and electrophoresis after DNA extraction from all samples and quality control. Finally, the PCR products were treated with RSal restriction enzyme and electrophoresed again, and the target polymorphism was checked.
Results:
The frequency of genotypes were TT=33.7%, CC=16.8% and CT=49.5% for healthy people and TT=43%, CC=14% and TC=43% in diabetics. The percentage of the T allele in healthy and diabetic people was as much as 42.1% and 64.5%, respectively.
Conclusion:
According to the results, there was probably a relationship between rs7903146 polymorphism in the TCF7L2 gene and diabetes.
Diabetes is characterized by decreased insulin sensitivity and is a growing health threat worldwide. Therefore, this study aimed to evaluate the relationship between rs7903146 polymorphism in the TCF7L2 gene with type 2 diabetes in East Azerbaijan province, Iran.
Materials and method:
A total of 100 blood samples were collected from diabetic and healthy people. The samples were subjected to specific primers, PCR, and electrophoresis after DNA extraction from all samples and quality control. Finally, the PCR products were treated with RSal restriction enzyme and electrophoresed again, and the target polymorphism was checked.
Results:
The frequency of genotypes were TT=33.7%, CC=16.8% and CT=49.5% for healthy people and TT=43%, CC=14% and TC=43% in diabetics. The percentage of the T allele in healthy and diabetic people was as much as 42.1% and 64.5%, respectively.
Conclusion:
According to the results, there was probably a relationship between rs7903146 polymorphism in the TCF7L2 gene and diabetes.
References
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